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Inducir completa Pólipo regeneración de la aboral Physa de la anémona de mar Starlet<em> Vectensis Nematostella</em
JoVE Journal
Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
Inducing Complete Polyp Regeneration from the Aboral Physa of the Starlet Sea Anemone Nematostella vectensis
DOI:

08:17 min

January 14, 2017

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Capitoli

  • 00:05Titolo
  • 01:05Selection and Relaxation of Nutritionally Conditioned Animals
  • 02:12Amputation
  • 03:44Assessing Regeneration with the Nematostella Regeneration Staging System (NRSS)
  • 05:47Results: Effects of Chloroquine, and Inhibitor of Autophagy
  • 06:35Conclusion

Summary

Traduzione automatica

Here we demonstrate how to induce and monitor regeneration in the Starlet Sea Anemone Nematostella vectensis, a model cnidarian anthozoan. We demonstrate how to amputate and categorize regeneration using a morphological staging system, and we use this system to reveal a requirement for autophagy in regenerating polyp structures.

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