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The Assembly and Application of ‘Shear Rings’: A Novel Endothelial Model for Orbital, Unidirectional and Periodic Fluid Flow and Shear Stress
JoVE Journal
Biologia
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JoVE Journal Biologia
The Assembly and Application of ‘Shear Rings’: A Novel Endothelial Model for Orbital, Unidirectional and Periodic Fluid Flow and Shear Stress
DOI:

09:20 min

October 31, 2016

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Capitoli

  • 00:05Titolo
  • 00:45Construction of 150-mm Diameter Shear Rings
  • 04:57Sterilization of Shear Rings
  • 06:31Shear Stress Studies
  • 07:40Results: Shear Rings Effectively Create Unidirectional and Periodic Shear Stress
  • 08:29Conclusion

Summary

Traduzione automatica

Different levels and patterns of fluid shear are known to modulate endothelial gene expression, phenotype and susceptibility to disease. We discuss the assembly and use of 'shear rings': a model that produces unidirectional, periodic shear stress patterns. Shear rings are simple to assemble, economical and can produce high cell yields.

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