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Immunologia e infezioni

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荧光激活细胞分选从小鼠骨髓浆树突状细胞的分离纯化

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Immunologia e infezioni

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JoVE Journal Immunologia e infezioni

Fluorescence-activated Cell Sorting for Purification of Plasmacytoid Dendritic Cells from the Mouse Bone Marrow

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荧光激活细胞分选从小鼠骨髓浆树突状细胞的分离纯化

DOI:

10.3791/54641-v

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08:01 min

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November 04, 2016

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Xiaofeng Liao, Melissa Makris, Xin M. Luo

¹Department of Biomedical Sciences and Pathobiology, Virginia-Maryland College of Veterinary Medicine,Virginia Polytechnic Institute and State University, ²Flow Cytometry Laboratory, Virginia-Maryland College of Veterinary Medicine,Virginia Polytechnic Institute and State University

Capitoli

00:05Titolo
00:49Bone Marrow Cell Isolation
02:30Cell Surface Staining for Fluorescence-Activated Cell Sorting (FACS)
04:09Plasmacytoid Cell FACS Purification
05:48Results: IFNalpha Production by FACS-Sorted pDC
07:10Conclusion

Summary

Traduzione automatica

我们使用荧光激活细胞分选来分离浆细胞样树突细胞（PDC）与来自狼疮易感小鼠的骨髓供的pDC的功能研究的高纯度报告的协议。

Tags

Keywords: Fluorescence-activated Cell Sorting Plasmacytoid Dendritic Cells Bone Marrow Lupus Immune Cell Interferon Alpha Density Gradient Cell Surface Staining FACS

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