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Visualizing the Early Stages of Phagocytosis
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Biologia
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JoVE Journal Biologia
Visualizing the Early Stages of Phagocytosis

Visualizing the Early Stages of Phagocytosis

DOI:
10.3791/54646-v

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08:04 min

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February 03, 2017

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, ,
1Department of Molecular Microbiology & Immunology,Oregon Health & Science University, 2Ragon Institute of MGH, MIT and Harvard

Capitoli

  • 00:05Titolo
  • 00:38Phagocytosis Binding Assay
  • 03:58Phagocytosis Uptake Assay
  • 04:50Live Imaging of Phagocytosis
  • 06:03Results: Sphingolipids Knockout Cells Show Reduced Phagocytosis
  • 07:03Conclusion

Summary

Traduzione automatica

Traduzione automatica

Here we describe a microscope-based technique to visualize and quantify the early cascades of events during phagocytosis of pathogens such as the fungi Candida albicans and particulates that are larger than 0.5 µm including zymosan and IgG-coated beads.

Tags

Keywords: PhagocytosisConfocal MicroscopePathogenHost CellVirusFluorescently Conjugated ZymosanDC 2.4 CellsEndocytosisCytosisCell Culture MediumMultiplicity Of InfectionCentrifugationParaformaldehydeAmmonium Chloride

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