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إعداد ألياف البروتين خارج الخلية مصفوفة لBrillouin الطيفي
JoVE Journal
Bioingegneria
This content is Free Access.
JoVE Journal Bioingegneria
Preparation of Extracellular Matrix Protein Fibers for Brillouin Spectroscopy
DOI:

07:19 min

September 15, 2016

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Capitoli

  • 00:05Titolo
  • 00:46Rat Tail Collagen Fiber Extraction
  • 02:16Bovine Nuchal Ligament Elastin Fiber Extraction
  • 03:44Reflective Substrate Fiber Mounting, Brillouin Experiment Setup and Fiber Spectra Acquisition
  • 04:55Results: Representative Micromechanics of Collagen and Elastin Fibers
  • 06:50Conclusion

Summary

Traduzione automatica

We present a protocol for the application of Brillouin light scattering spectroscopy to elastin and trypsin-purified type I collagen fibers of the extracellular matrix to extract their full elastic properties.

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