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अर्द्ध मात्रात्मक, मध्यम throughput माइक्रोबैक्टीरिया की गणना के लिए लकड़ी का कोयला अग्रवाल Resazurin परख के दृश्य
JoVE Journal
Immunologia e infezioni
This content is Free Access.
JoVE Journal Immunologia e infezioni
Visualization of the Charcoal Agar Resazurin Assay for Semi-quantitative, Medium-throughput Enumeration of Mycobacteria
DOI:

09:57 min

December 14, 2016

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Capitoli

  • 00:05Titolo
  • 00:46Preparation of CARA Microplates
  • 02:20Setting up Replicating and Non-replicating MIC90 Assays
  • 04:23Inoculation of CARA Microplates
  • 05:30Developing CARA Microplates and Data Analysis
  • 07:22Results: Enumeration of Mycobacteria via the CARA Assay
  • 09:06Conclusion

Summary

Traduzione automatica

The charcoal agar resazurin assay (CARA) is a semi-quantitative, medium-throughput method to assess activity of test agents against mycobacteria that are replicating, non-replicating, or both. The CARA permits rapid evaluation of time- and concentration-dependent activity and identifies parameters to pursue by colony forming unit (CFU) assays.

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