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LED热流量 - 结合光遗传学与流式细胞仪

JoVE Journal
Biologia

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JoVE Journal Biologia

LED Thermo Flow — Combining Optogenetics with Flow Cytometry

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LED热流量 - 结合光遗传学与流式细胞仪

DOI:

10.3791/54707-v

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05:49 min

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December 30, 2016

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Kathrin Brenker^2,3, Kerstin Osthof⁴, Jianying Yang³, Michael Reth^3,5

¹Max-Planck Institute for Immunobiology und Epigenetics, ²Spemann Graduate School of Biology and Medicine (SGBM),University of Freiburg, ³Centre for Biological Signaling Studies, BIOSS,University of Freiburg, ⁴Albert-Ludwigs-Universität, ⁵Institute for Biology III (Mol. Immunology),Albert-Ludwigs-Universität

Capitoli

00:05Titolo
00:33Estimation of Required Light Intensity
02:09Measuring the Kinetics of an Optogenetic Tool
03:28Results: Photoswitching of the Dronpa-Linker-Dronpa Protein
04:57Conclusion

Summary

Traduzione automatica

Optically controlled substances are powerful tools to study signaling pathways. To expand the spectrum of possible experiments, we developed a device for studying optically controlled substances in real time using flow cytometry: the LED Thermo Flow.

Tags

LED Thermo Flow Optogenetics Flow Cytometry Cell Signaling Optically Controlled Substances Dronpa Photoswitching Ramos B Lymphocytes 400 Nm LED 500 Nm LED Glass FACS Tubes Fluorescence Intensity

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