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利用RNA测序来检测新剪接变异体在相关耐药<em>体外</em>癌症模型
JoVE Journal
Ricerca sul cancro
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JoVE Journal Ricerca sul cancro
Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models
DOI:

09:58 min

December 09, 2016

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Capitoli

  • 00:05Titolo
  • 01:09Characterization of Drug Resistance Profiles through Cytotoxicity Assays
  • 05:01RNA Isolation and Library Preparation for RNA-sequencing
  • 05:55Validation of the Results by RT-PCR and qRT-PCR Assays
  • 07:21Results: Detection of Differential Splicing Events in Chemotherapy-resistant Cell Lines Compared to Parental Wild-type by Using RNA-sequencing
  • 09:07Conclusion

Summary

Traduzione automatica

这里,我们描述旨在调查异常剪接的耐药性在实体瘤和血液恶性肿瘤的影响的协议。为了这个目标,我们通过RNA测序分析,家长和体外抗车型的转录配置文件,并建立了定量RT-PCR为基础的方法来验证候选基因。

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