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分析蛋白导入进强调，从植物中分离叶绿体

JoVE Journal
Biologia

This content is Free Access.

JoVE Journal Biologia

Analysis of Protein Import into Chloroplasts Isolated from Stressed Plants

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分析蛋白导入进强调，从植物中分离叶绿体

DOI:

10.3791/54717-v

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10:18 min

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November 01, 2016

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Qihua Ling, Paul Jarvis

¹Department of Plant Sciences,University of Oxford

Capitoli

00:05Titolo
00:46Growth of Arabidopsis Plants and the Stress Treatment
01:34Chloroplast Isolation
05:06Analysis of the Yield and the Intactness of Chloroplasts
06:19Chloroplast Protein Import
08:04Results: Chloroplast Import of the PsaD Protein Is Elevated in the sp1 Mutant under Stress Conditions
09:12Conclusion

Summary

Traduzione automatica

在这里，我们描述了一个新的方法来研究蛋白导入叶绿体中分离的压力下。该方法是快速和简单的，并且可以应用于研究的叶绿体蛋白质导入不同胁迫条件的后果，以及相应的调节机制。

Tags

Protein Import Chloroplasts Stressed Plants Arabidopsis Thaliana Protein Import Regulation Chloroplast Isolation Percoll Gradient Protein Import Assay

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