È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.
Capitoli
- 00:05Titolo
- 00:34Compound Microscope Setup
- 02:07Acquiring Images with a Compound Microscope
- 03:19Dissection Microscopy Setup
- 04:31Acquiring Images with a Dissection Microscope
- 08:51Results: Cell Counting Accuracy and Value of Calibration
- 10:12Conclusion
This paper describes the quantification of hemocytometer and migration/invasion micrographs through two new open-source ImageJ plugins Cell Concentration Calculator and migration assay Counter. Furthermore, it describes image acquisition and calibration protocols as well as discusses in detail the input requirements of the plugins.
