Rivista
/
Immunologia e infezioni
/
A Simple Fluorescence Assay for Quantification of Canine Neutrophil Extracellular Trap Release
JoVE Journal
Immunologia e infezioni
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Immunologia e infezioni
A Simple Fluorescence Assay for Quantification of Canine Neutrophil Extracellular Trap Release

A Simple Fluorescence Assay for Quantification of Canine Neutrophil Extracellular Trap Release

DOI:
10.3791/54726-v

•

06:45 min

•

November 21, 2016

•

, ,
1Department of Veterinary Microbiology and Preventative Medicine, College of Veterinary Medicine,Iowa State University, 2MRC Centre for Inflammation Research,University of Edinburgh, 3Department of Veterinary Clinical Sciences, College of Veterinary Medicine,Iowa State University

Capitoli

  • 00:05Titolo
  • 00:42Neutrophil Isolation
  • 03:41DNA Release Assay
  • 04:44Risultati
  • 05:42Conclusion

Summary

Traduzione automatica

Traduzione automatica

Neutrophil extracellular traps (NETs) are networks of DNA, histones and neutrophil proteins. Although a component of the innate immune response, NETs are implicated in autoimmunity and thrombosis. This protocol describes a simple method for canine neutrophil isolation and quantification of NETs using a microplate fluorescence assay.

Tags

Neutrophil Extracellular TrapFluorescence AssayCanineNeutrophil IsolationAgonistImmunologyVeterinary MedicineCell DeathCell SeparationDextranPolysucroseSodium DiatrizoateErythrocyte LysisPBS

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

Neutrophil Extracellular Traps: How to Generate and Visualize Them

In vitro Coculture Assay to Assess Pathogen Induced Neutrophil Trans-epithelial Migration

In Vitro Canine Neutrophil Extracellular Trap Formation: Dynamic and Quantitative Analysis by Fluorescence Microscopy

Quantification of Efferocytosis by Single-cell Fluorescence Microscopy

Automated Image-Based Quantification of Neutrophil Extracellular Traps Using NETQUANT

A High-throughput Assay to Assess and Quantify Neutrophil Extracellular Trap Formation

In Vitro Stimulation and Visualization of Extracellular Trap Release in Differentiated Human Monocyte-derived Macrophages

Humanized Mediator Release Assay as a Read-Out for Allergen Potency

Quantifying Myeloperoxidase-DNA and Neutrophil Elastase-DNA Complexes from Neutrophil Extracellular Traps by Using a Modified Sandwich ELISA

Author Spotlight: Balancing Speed, Cost, and Accuracy in Neutrophil Function Assessment with NeutroFun Screen Protocol

Read Article