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기능 유전체학에게 검사를 활용하여 암 세포 회전 타원체 문화에 잠재적으로 소설 약 표적을 식별하는
JoVE Journal
Ricerca sul cancro
This content is Free Access.
JoVE Journal Ricerca sul cancro
Utilizing Functional Genomics Screening to Identify Potentially Novel Drug Targets in Cancer Cell Spheroid Cultures
DOI:

07:48 min

December 26, 2016

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Capitoli

  • 00:05Titolo
  • 01:17Reverse Transfection of BT474 Cells
  • 03:51Automated Image Acquisition
  • 05:23Results: Functional Genomic Investigation of BT474 Spheroids Uncovers Oncogenic Dependencies
  • 07:02Conclusion

Summary

Traduzione automatica

Identifying novel drug targets that transition from pre-clinical testing to human trials is a scientific priority. To that end, here we describe a functional genomics approach for examining the impact of gene depletion on cancer cell line spheroids, which more appropriately model human cancers in vivo.

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