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Molecular Diffusion in Plasma Membranes of Primary Lymphocytes Measured by Fluorescence Correlation Spectroscopy
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Molecular Diffusion in Plasma Membranes of Primary Lymphocytes Measured by Fluorescence Correlation Spectroscopy

Molecular Diffusion in Plasma Membranes of Primary Lymphocytes Measured by Fluorescence Correlation Spectroscopy

DOI:
10.3791/54756-v

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12:06 min

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February 01, 2017

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, ,
1Department of Microbiology, Tumor and Cell Biology,Karolinska Institutet

Capitoli

  • 00:05Titolo
  • 01:07Starting the FCS System
  • 02:26Pinhole Adjustment
  • 04:41Measure the Transit Time of the Free Fluorophore
  • 05:50Measure the Transit Time of Free Antibody
  • 06:51Cell Measurements
  • 09:47Results: Assessing the Autocorrelation Curve
  • 11:04Conclusion

Summary

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A method to measure protein diffusion in membranes of primary immune cells using fluorescence correlation spectroscopy (FCS) is described. In this paper, the use of antibodies for fluorescent labeling is illustrated.

Tags

Molecular DiffusionPlasma MembranesPrimary LymphocytesFluorescence Correlation SpectroscopyImmunologyCell BiologyNatural Killer CellsMembrane Receptor DynamicsImmunotherapyCancerPrimary CellsCell LinesConfocal Laser Scanning MicroscopeFCS MeasurementsFluorophoresDyePoly-L-lysineCoverglass SlideObjective Lens

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