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一个优化的协议通过使用金纳米粒子的放射性标记效率<sup> 125</sup>我标记的叠氮辅基

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Chimica

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JoVE Journal Chimica

An Optimized Protocol for the Efficient Radiolabeling of Gold Nanoparticles by Using a ¹²⁵I-labeled Azide Prosthetic Group

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一个优化的协议通过使用金纳米粒子的放射性标记效率<sup> 125</sup>我标记的叠氮辅基

DOI:

10.3791/54759-v

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07:23 min

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October 10, 2016

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Jongho Jeon², Ha Eun Shim, Sajid Mushtaq², Mi Hee Choi, Sang Hyun Park², Dae Seong Choi, Beom-Su Jang²

¹Advanced Radiation Technology Institute,Korea Atomic Energy Research Institute, ²Department of Radiation Biotechnology and Applied Radioisotope Science,University of Science and Technology

Capitoli

00:05Titolo
01:08Radiosynthesis of the ¹²⁵I-labeled Azide Prosthetic Group
03:59Radiolabeling of Dibenzocyclooctyne-group-modified Gold Nanoparticles via the Copper-free Click Reaction
05:53Results: Validation of the Purified Radiolabeled Products
06:32Conclusion

Summary

Traduzione automatica

对于一个¹²⁵ I标记的叠氮化物的合成和dibenzocyclooctyne（DBCO） -基共轭，13纳米大小的金使用无铜点击反应的纳米粒子的放射性标记的详细过程进行说明。

Tags

Keyword Extraction:Radiolabeling Gold Nanoparticles 125I-labeled Azide Strain Promoted Copper Free Click Reaction Radiochemistry Radio Isotope Labeled Imaging Probes PET/SPECT Radiochemical Yield Radiochemical Purity Radio Iodination Reaction Chloramine T Sodium Metabisulfite HPLC Analysis Reversed Phase Analytical Radio HPLC Preparative Radio HPLC Radiochemical Yield

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