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Flow Cytometric Analysis of Natural Killer Cell Lytic Activity in Human Whole Blood
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Flow Cytometric Analysis of Natural Killer Cell Lytic Activity in Human Whole Blood

Flow Cytometric Analysis of Natural Killer Cell Lytic Activity in Human Whole Blood

DOI:
10.3791/54779-v

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08:14 min

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March 17, 2017

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, , , ,
1Center for Excellence in Post-Harvest Technologies,North Carolina A&T State University, North Carolina Research Campus, 2Human Performance Laboratory,Appalachian State University, North Carolina Research Campus

Capitoli

  • 00:05Titolo
  • 00:44Automated NK Cell Separation
  • 02:17Cytotoxicity Assay Sample Preparation
  • 03:36Imaging Flow Cytometer Acquisition and Analysis
  • 06:25Results: Representative NK Cell Lytic Activity Analyses
  • 07:41Conclusion

Summary

Traduzione automatica

Traduzione automatica

This work describes an advanced workflow for the accurate and fast determination of NK (Natural Killer) cell count and NK cell cytotoxicity in human blood samples.

Tags

Keywords: Flow CytometryNatural Killer (NK) CellsCytotoxicityWhole BloodCell SeparationK562 CellsPropidium IodideImmunology

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