Waiting
Elaborazione accesso...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Developmental Biology / Analysis of Coronary Vessels in Cleared Embryonic …
JoVE Journal
Developmental Biology

This Contenuto is Open Access.

JoVE Journal Developmental Biology
Analysis of Coronary Vessels in Cleared Embryonic Hearts
 

Analysis of Coronary Vessels in Cleared Embryonic Hearts

Article DOI: 10.3791/54800-v • 08:25 min • December 7th, 2016
December 7th, 2016 •
1, 1, 2, 1
1Developmental Biology of Birth Defects, UCL Institute of Child Health, 2MRC Centre for Regenerative Medicine, SCRM Building, University of Edinburgh

Capitoli

Riepilogo
Automatic Translation

We present a protocol for the analysis of coronary vessels in whole embryonic murine hearts up to E15.5, using standard immunological staining methods followed by optical clearance and confocal microscopy. This technique enables visualization of blood vessels throughout the entire heart without the need for time-consuming analysis of serial sections.

Tags

BABB Base Tissue Clearing Method Coronary Vessels Cleared Embryonic Hearts Non-invasive Imaging Cardiovascular Development Coronary Formation Tissue Clearance Methods Therapy Of Coronary Heart Disease Vessel Formation Preventing Disease Restoring Cardiac Function Dissecting Microscope Forceps Eutherian Sacs Timed Pregnant Mouse Yolk Sac Umbilical Cord Stainless Steel Minutemen Pins Silicon Elastomer Coated 35mm Petri Dish Fine Forceps Great Vessels Interior Organ Removal Fresh 35mm Petri Dish PBS
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter