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JoVE Journal Neuroscience
Preparation of Primary Mixed Glial Cultures from Adult Mouse Spinal Cord Tissue
 

Preparation of Primary Mixed Glial Cultures from Adult Mouse Spinal Cord Tissue

Article DOI: 10.3791/54801-v • 07:13 min • November 19th, 2016
November 19th, 2016 •
1, 1
1Department of Biomedical Sciences, College of Osteopathic Medicine, University of New England

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The development of neuropathic pain involves pathological changes of spinal cord glial cells. A reliable glial culture system derived from adult spinal cord tissue and designed for studying these cells in vitro is lacking. Therefore, we show here how to establish primary mixed glial cultures from adult mouse spinal cord tissue.

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Keywords: Primary Mixed Glial Cultures Adult Mouse Spinal Cord In Vitro Studies Neuropathic Pain Multiple Sclerosis Tissue Culture HBSS Papain DNAse Enzyme Digestion Single Cell Suspension Albumin Ovomucoid Inhibitor DNAse Centrifugation Myelin Removal
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