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Preparation of Primary Mixed Glial Cultures from Adult Mouse Spinal Cord Tissue
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Capitoli
- 00:05Titolo
- 00:55Preparation of the Single Cell Suspension
- 03:12Removal of Myelin from the Single Cell Suspension
- 05:03Results: Mixed Glial Cultures and Response to Stimuli
- 06:11Conclusion
The development of neuropathic pain involves pathological changes of spinal cord glial cells. A reliable glial culture system derived from adult spinal cord tissue and designed for studying these cells in vitro is lacking. Therefore, we show here how to establish primary mixed glial cultures from adult mouse spinal cord tissue.
