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An Alternative and Validated Injection Method for Accessing the Subretinal Space via a Transcleral Posterior Approach
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Neuroscienze
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JoVE Journal Neuroscienze
An Alternative and Validated Injection Method for Accessing the Subretinal Space via a Transcleral Posterior Approach

An Alternative and Validated Injection Method for Accessing the Subretinal Space via a Transcleral Posterior Approach

DOI:
10.3791/54808-v

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07:13 min

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December 07, 2016

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, , , , ,
1Jules Stein Eye Institute,University of California, Los Angeles

Capitoli

  • 00:05Titolo
  • 00:50Injection Site Preparation
  • 02:41Sclerotomy and Subretinal Injection
  • 04:40Results: Retinal Structure Was Assessed Using OCT Imaging
  • 06:29Conclusion

Summary

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Subretinal injections are the most common technique for delivering large therapeutic agents such as proteins and viral vectors to photoreceptors and the retinal pigment epithelium. An alternative method in mice that successfully targets the subretinal space with minimal collateral damage and fast recovery times is described here.

Tags

Keywords: Subretinal InjectionTranscleral Posterior ApproachViral VectorsPharmacological AgentsPluripotent Stem CellsRetinal DegenerationFluoresceinAnesthetized MousePupil DilationScleral IncisionOphthalmic Blade33-gauge NeedleMinimally InvasiveRetinal Structure And Function

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