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Het visualiseren van de Interrenal steroïdogene Tissue en haar Vasculaire Microenvironment in zebravis
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Capitoli
- 00:05Titolo
- 01:04Whole Mount 3β-Hsd Activity Staining
- 03:15IHC Analysis of 3β-Hsd Activity-stained Embryos
- 05:28Results: Zebrafish Interrenal Steroidogenic Tissue
- 06:30Conclusion
De interrenal klier in de zebravis is de teleostean tegenhanger van de zoogdieren bijnier. Dit protocol wordt beschreven hoe de 3-β-hydroxysteroïdedehydrogenase (Δ 5-4 isomerase, 3β-Hsd) verricht enzymatische activiteit test, die gedifferentieerde steroïdogene cellen in zebravisembryo detecteert.
Tags
Vascular MicroenvironmentZebrafishEnzymatic Activity AssayWhole-mount StainingTransgenic Fluorescent Reporter LinesVibratome SectioningImmunohistochemistryConfocal Microscopy3beta-Hsd Activity StainingPigment InhibitionPFA FixationPBST WashingChromogenic Signal DetectionPrecipitationPost-fixationGlycerol ClearingWhole-mount Microscopy
