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头孢哌酮治疗小鼠模型的临床相关的<em>艰难梭菌</em>应变R20291

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Immunologia e infezioni

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JoVE Journal Immunologia e infezioni

Cefoperazone-treated Mouse Model of Clinically-relevant Clostridium difficile Strain R20291

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头孢哌酮治疗小鼠模型的临床相关的<em>艰难梭菌</em>应变R20291

DOI:

10.3791/54850-v

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06:51 min

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December 10, 2016

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Jenessa A. Winston, Rajani Thanissery, Stephanie A. Montgomery, Casey M. Theriot

¹Department of Population Health and Pathobiology,North Carolina State University College of Veterinary Medicine, ²Department of Pathology and Laboratory Medicine, Lineberger Comprehensive Cancer Center,University of North Carolina School of Medicine

Capitoli

00:05Titolo
00:53Inoculation of Mice with Clostridium difficile
03:22Enumeration of C. difficile from Mouse Fecal Content
05:03Results: The Murine Model of C. difficile Infection Demonstrates Significant Weight Loss, Bacterial Colonization and Histopathological Changes in Infected Mice
06:10Conclusion

Summary

Traduzione automatica

该协议概述了使用临床相关和转听话应变，R20291 艰难梭菌感染（CDI）的头孢哌酮小鼠模型。临床疾病的监测， 艰难梭菌细菌枚举，毒素的细胞毒作用，并在小鼠模型中在整个CDI病理学改变强调在协议中详述。

Tags

Keywords: Clostridium Difficile Mouse Model Cefoperazone Spore Inoculum Gavage Anaerobic Dilution Enumeration Toxin Cytotoxicity Histopathology

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