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ACT-PRESTO：生物组织清算和体积成像方法免疫标记
JoVE Journal
Biologia
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JoVE Journal Biologia
ACT-PRESTO: Biological Tissue Clearing and Immunolabeling Methods for Volume Imaging
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ACT-PRESTO：生物组织清算和体积成像方法免疫标记

DOI:
10.3791/54904-v

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07:46 min

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December 31, 2016

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1Department of Anatomy,Korea University College of Medicine

Capitoli

  • 00:05Titolo
  • 00:43Hydrogel Monomer Infusion and Polymerization
  • 01:42Electrophoretic Tissue Clearing
  • 02:37Immunolabeling of Dense Tissues
  • 05:24Results: PRESTO Immunolabeling of ACT-cleared Dense Tissue
  • 06:27Conclusion

Summary

Traduzione automatica

Traduzione automatica

ACT-PRESTO（大分子活性清晰度技术压力相关的有效和稳定的转印到器官）实现快速，高效，但廉价的组织清算和快速抗体渗透到通过被动扩散或压力辅助输送清除组织。使用这种方法，广泛的组织中可以清除，由多个抗体免疫标记，和体积成像。

Tags

ImmunolabelingVolume ImagingNeuronal ConnectionsMolecular CategorizationStructural ChangeSub-cellular AnalysisHydrogelPolymerizationC-PRESTO (centrifugal-assisted Protein Retention Encoding Through Stochastic Trafficking)S-PRESTO (stochastic Protein Retention Encoding Through Stochastic Trafficking)

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