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Chondrogenic Differentiation Induction of Adipose-derived Stem Cells by Centrifugal Gravity
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Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
Chondrogenic Differentiation Induction of Adipose-derived Stem Cells by Centrifugal Gravity

Chondrogenic Differentiation Induction of Adipose-derived Stem Cells by Centrifugal Gravity

DOI:
10.3791/54934-v

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08:30 min

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February 24, 2017

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, ,
1CiSTEM Laboratory, Convergent Research Consortium for Immunologic Disease, Division of Rheumatology, Seoul St. Mary’s Hospital, College of Medicine,The Catholic University of Korea

Capitoli

  • 00:05Titolo
  • 00:54Centrifugal Gravity Loading
  • 02:22Pellet Culture
  • 03:12Micromass Culture
  • 04:10Paraffin-embedding Cell Pellets
  • 05:59Results: Upregulation of Chondrogenic Differentiation Markers in Adipose-derived Stem Cells Following Centrifugal Gravity Loading
  • 07:59Conclusion

Summary

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Mechanical stress can induce the chondrogenic differentiation of stem cells, providing a potential therapeutic approach for the repair of impaired cartilage. We present a protocol to induce the chondrogenic differentiation of adipose-derived stem cells (ASCs) using centrifugal gravity (CG). CG-induced upregulation of SOX9 results in the development of chondrogenic phenotypes.

Tags

Chondrogenic DifferentiationAdipose-derived Stem CellsCentrifugal GravityCartilage RepairPrechondrogenic DifferentiationOsteogenic DifferentiationFibrogenic DifferentiationCytokinesEDTAHemocytometerMicromass CultureChondrogenic Differentiation MediumTGF-beta-1

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