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可追踪记者乳腺癌患者来源的移植瘤贴标肿瘤生长和转移的研究

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Labeling of Breast Cancer Patient-derived Xenografts with Traceable Reporters for Tumor Growth and Metastasis Studies

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可追踪记者乳腺癌患者来源的移植瘤贴标肿瘤生长和转移的研究

DOI:

10.3791/54944-v

•

09:53 min

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November 30, 2016

•

Colton Hanna, Letty Kwok, Jessica Finlay-Schultz, Carol A. Sartorius, Diana M. Cittelly

¹Department of Pathology, School of Medicine,University of Colorado Anschutz Medical Campus

Capitoli

00:05Titolo
00:58Dissection and Dissociation of Patient-derived Xenograft (PDX)-derived Tumor Cells
03:20Human Epithelial Cancer Cell Enrichment
05:13PDX-derived Tumor Cell Transduction, Transduction Efficiency Evaluation and Tumor Cell Re-implantation
06:48Results: PDX-dissociated Breast Cancer Cell Transduction Using High Titer Lentiviral Vectors
08:33Conclusion

Summary

Traduzione automatica

我们描述了源自患者的异种移植物（PDXs）用表达绿色荧光蛋白和荧光素酶记者慢病毒颗粒的稳定标记的方法。该方法允许用于跟踪PDXs的生长在主站点，以及检测使用体内成像系统自发和实验转移。

Tags

Keywords: Breast Cancer Patient-derived Xenografts PDX Reporter Genes Tumor Growth Metastasis In Vivo Imaging Aseptic Technique Cell Isolation Red Blood Cell Lysis

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