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生活多巴胺能神经元可靠的鉴定中脑培养使用RNA测序和TH-启动子驱动的EGFP表达
JoVE Journal
Neuroscienze
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JoVE Journal Neuroscienze
Reliable Identification of Living Dopaminergic Neurons in Midbrain Cultures Using RNA Sequencing and TH-promoter-driven eGFP Expression
DOI:

10:54 min

February 10, 2017

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Capitoli

  • 00:05Titolo
  • 00:59Isolation of Embryonic Midbrain
  • 02:55Dissociation and Plating of Cells
  • 05:34Harvesting Cultured Dopaminergic Neurons for RNA Sequencing
  • 07:04Generation, Purification and Amplification of First-strand cDNA
  • 09:13Results: RNA-seq Detection of Expressed Transcripts in Single Dopaminergic Neurons
  • 09:39Conclusion

Summary

Traduzione automatica

帕金森病(PD),黑质(SNC)多巴胺能神经元退化,导致运动功能障碍。此处我们报告从表达eGFP的小鼠通过酪氨酸羟化酶(TH)的启动子序列驱动的培养腹侧中脑神经元,从培养收获个别荧光神经元,并使用RNA-SEQ测量它们转录的协议。

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