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Production of Replication-Defective Retrovirus by Transient Transfection of 293T cells
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Biologia
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JoVE Journal Biologia
Production of Replication-Defective Retrovirus by Transient Transfection of 293T cells

Production of Replication-Defective Retrovirus by Transient Transfection of 293T cells

DOI:
10.3791/550-v

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09:40 min

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December 04, 2007

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,
1Molecular Oncology Research Institute,Tufts University

Capitoli

  • 00:08Introduction
  • 00:51Preparation of 293T cells
  • 03:51Preparation of transfection mixture
  • 04:52Transfection
  • 07:02Collection of virus
  • 08:00Discussion

Summary

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This technique demonstrates an efficient way to prepare replication-defective retroviral stocks encoding a human oncogene, and subsequently used for induction of myeloproliferative disease in the mouse model.

Tags

Replication-defective RetrovirusTransient Transfection293T CellsHuman Myeloproliferative DiseasesOncogenesBcr-AblGrowth Factor Receptor-derived OncogenesZNF198-FGFR1Bcr-PDGFRαMouse ModelBone Marrow CellsRetroviral VectorGag-pol-env Packaging FunctionsCalcium Phosphate TransfectionChloroquineEcotropic VirusCulture SupernatantCell-linesTransformation StudiesIn Vitro StudiesPrimary CellsMouse Model

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