È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.
1Bioengineering Program,Lehigh University, 2Center for Photonics and Nanoelectronics,Lehigh University, 3Department of Electrical and Computer Engineering,Lehigh University, 4State Key Laboratory of Software Engineering,Wuhan University, 5Genetics and Aging Research Unit, Department of Neurology,Massachusetts General Hospital and Harvard Medical School
Capitoli
- 00:05Titolo
- 01:08Mounting Larva for OCM Imaging
- 02:13Imaging Larval Hearts with OCM
- 04:39Imaging at the PD1 Stage
- 05:52Imaging at the PD2 to PD5 Stages
- 07:25Imaging at the Adult Stage
- 08:34Results: Heart Rate during Development
- 09:36Conclusion
Here, the experimental protocols are described for preparing Drosophila at different developmental stages and performing longitudinal optical imaging of Drosophila heartbeats using a custom optical coherence microscopy (OCM) system. The cardiac morphological and dynamical changes can be quantitatively characterized by analyzing the heart structural and functional parameters from OCM images.
