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The ChIP-exo Method: Identifying Protein-DNA Interactions with Near Base Pair Precision
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Biologia
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JoVE Journal Biologia
The ChIP-exo Method: Identifying Protein-DNA Interactions with Near Base Pair Precision

The ChIP-exo Method: Identifying Protein-DNA Interactions with Near Base Pair Precision

DOI:
10.3791/55016-v

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09:27 min

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December 23, 2016

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1Department of Molecular Physiology and Biophysics,Vanderbilt University

Capitoli

  • 00:05Titolo
  • 00:41Sonication of Nuclear Lysates
  • 02:31Chromatin Immunoprecipitation
  • 03:55P7 Adapter Ligation and Lambda Exonuclease Reactions
  • 05:06RecJf Nuclease, Crosslink Reversal, and P5 Adapter Ligation Reactions
  • 06:47Results: Chromatin Immunoprecipitation Exo-mediated Protein-DNA Interactions
  • 08:21Conclusion

Summary

Traduzione automatica

Traduzione automatica

Here, we present a protocol to achieve near base pair resolution of protein-DNA interactions. This is obtained by exonuclease treatment of DNA fragments selectively enriched by chromatin immunoprecipitation (ChIP-exo) followed by high throughput sequencing.

Tags

ChIP-exoProtein-DNA InteractionsGene RegulationEpigeneticsTranscription MachineryChromatinSonicationNuclear LysatesChromatin ImmunoprecipitationChIP ReactionAntibody Bead Conjugates

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