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Capitoli
- 00:05Titolo
- 00:57Collection and Fixation of Developing and Fully Formed Plant Structures
- 02:04Dissection and Dehydration of Developing and Fully Formed Plant Structures
- 03:02Mounting and Preparing Plant Tissues for SEM Observation of Developing and Fully Formed Plant Structures
- 04:20Study of Cyst Behavior of Saprolegnia (Oomycetes) on Different Surfaces
- 06:04Study of Herbarium Fungal Spores of Phellorinia herculanea under SEM
- 07:20Results: Recovery of Plant Development, Differential Spine Growth of Saprolegnia parasitica, and Nurse Cells in Spores of Phellorinia herculanea f. stellata
- 09:01Conclusion
Problems in the processing of biological samples for scanning electron microscopy observation include cell collapse, treatment of samples from wet microenvironments and cell destruction. Low-cost and relatively rapid protocols suited for preparing challenging samples such as floral meristems, oomycete cysts, and fungi (Agaricales) are compiled and detailed here.
