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Règlement de Picornavirus Tropisme base microARN-
JoVE Journal
Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
MicroRNA-based Regulation of Picornavirus Tropism
DOI:

09:05 min

February 06, 2017

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Capitoli

  • 00:05Titolo
  • 01:04Titrating Virus Stocks by Calculating 50% Tissue-culture Infectious Dose (TCID50)
  • 02:33Evaluating MicroRNA-targeting Efficacy: Single-step Growth Kinetics
  • 04:29Evaluating MicroRNA-targeting Specificity: Virus-spreading Assay Using Synthetic MicroRNA Mimics
  • 07:01Results: Comparing MicroRNA-targeted Virus to Unmodified Virus
  • 08:32Conclusion

Summary

Traduzione automatica

We describe here a method for regulating picornavirus tropism by incorporating sequences complementary to specific microRNAs into the viral genome. This protocol can be adapted to all different classes of viruses with modifications based upon the length and nature of their life cycle.

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