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מיקרוסקופ פלואורסצנטי גיליון אור של שורשי הצמח גדל על פני השטח של ג'ל
JoVE Journal
Biologia
Author Produced
This content is Free Access.
JoVE Journal Biologia
Light Sheet Fluorescence Microscopy of Plant Roots Growing on the Surface of a Gel
DOI:

06:41 min

January 18, 2017

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Capitoli

  • 00:00Titolo
  • 01:24Plant Cultivation (Arabidopsis thaliana seedlings)
  • 02:04Plant Sample Preparation
  • 03:28Microscope Setup
  • 05:18Risultati
  • 06:00Conclusion

Summary

Traduzione automatica

This protocol shows a plant sample preparation method for light-sheet microscopy. The setup is characterized by mounting the plant vertically on the surface of a gel and letting it grow in controlled bright conditions. This allows long-term observation of plant organ development in standardized conditions.

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