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制备及文化活动从成年和老年人类骨骼肌成肌前体细胞/成肌细胞主要的
JoVE Journal
Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
Preparation and Culture of Myogenic Precursor Cells/Primary Myoblasts from Skeletal Muscle of Adult and Aged Humans
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制备及文化活动从成年和老年人类骨骼肌成肌前体细胞/成肌细胞主要的

DOI:
10.3791/55047-v

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10:10 min

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February 16, 2017

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, , ,
1Institute of Ageing and Chronic Disease,University of Liverpool, 2Aintree University Hospital

Capitoli

  • 00:05Titolo
  • 00:47Culture Preparation
  • 01:40Tissue Digestion: Mechanical and Enzymatic Dissociation
  • 04:16Seeding of Cells
  • 05:29Culture and Passaging of Cells
  • 07:52Results: Human Primary Myoblasts Characterization and Its Use for Functional Studies of Muscle Homeostasis In Vitro
  • 09:16Conclusion

Summary

Traduzione automatica

Traduzione automatica

本协议描述的隔离和成肌细胞祖细胞培养的成年和老年人的骨骼肌肉健壮，重复性好，简单的方法。这里使用的肌肉包括脚和腿部的肌肉。这种方法使对于功能研究初级成肌细胞的富集群体的分离。

Tags

Keywords: Myogenic Precursor CellsPrimary MyoblastsSkeletal MuscleAdultAgedIsolationIn Vitro ModelMuscle HomeostasisAgingLamininEnzymatic DigestionDMEMDPBSEthanolGrowth MediaCell StrainerCentrifugation

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