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栽培<em>秀丽隐杆线虫</em>在三个维度实验室
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Biologia
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JoVE Journal Biologia
Cultivation of Caenorhabditis elegans in Three Dimensions in the Laboratory
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栽培<em>秀丽隐杆线虫</em>在三个维度实验室

DOI:
10.3791/55048-v

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09:01 min

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December 12, 2016

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, ,
1Division of Biological Science and Technology,Yonsei University

Capitoli

  • 00:05Titolo
  • 00:52Prepare Solutions and Bacteria Culture for NGT-3D and NGB-3D
  • 02:23Making NGT-3D and NGB-3D
  • 05:18Measure Fitness of Worm Population on NGT-3D
  • 06:47Results: Development, Fertility, and Lifespan of C. elegans Cultivated in NGT-3D Is Comparable with That of the Standard 2D NGM
  • 08:12Conclusion

Summary

Traduzione automatica

Traduzione automatica

我们提出了一个简单的方法来构建三维线虫培养系统称为NGT-3D和NGB-3D。这些可以被用来研究在更类似于天然线虫栖息比标准2D实验室线虫培养板栖息线虫健身和行为。

Tags

Keywords: 3D CultivationNematode BehaviorCaenorhabditis ElegansNatural ConditionsBacterial CultureSerial DilutionAgar MediaOtto ClaveSterile SolutionsCholesterol Solution

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