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Isolation and Characterization of Microvesicles from Peripheral Blood
JoVE Journal
Biologia
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JoVE Journal Biologia
Isolation and Characterization of Microvesicles from Peripheral Blood

Isolation and Characterization of Microvesicles from Peripheral Blood

DOI:
10.3791/55057-v

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06:03 min

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January 06, 2017

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, , , ,
1Department of Hematology/Medical Oncology,University Medical Center Göttingen

Capitoli

  • 00:05Titolo
  • 00:47Microvesicle (MV) Isolation
  • 02:26MV Flow Cytometric Characterization
  • 03:31Results: Representative Blood-isolated MV Characterization
  • 05:24Conclusion

Summary

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Extracellular vesicles present in blood have been suggested as novel biomarkers for various diseases. Here, we present a protocol for the isolation of large plasma membrane-derived microvesicles from peripheral blood samples and their subsequent analysis by conventional flow cytometry and Western Blotting.

Tags

Keywords: MicrovesiclesPeripheral BloodPlasmaCentrifugationFlow CytometryCharacterizationProtein ConcentrationProinvasiveMetastasisTumor PatientsEDTAPBSFCSAntibody

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