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一个单一的鼠标骨骼肌的相邻区Cryosectioning的基因表达和组织学分析
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Biologia
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JoVE Journal Biologia
Cryosectioning of Contiguous Regions of a Single Mouse Skeletal Muscle for Gene Expression and Histological Analyses
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一个单一的鼠标骨骼肌的相邻区Cryosectioning的基因表达和组织学分析

DOI:
10.3791/55058-v

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08:17 min

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December 12, 2016

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1Department of Pharmaceutical and Biomedical Sciences,University of Georgia

Capitoli

  • 00:05Titolo
  • 00:50Cryopreservation
  • 02:04Collecting Cryosections for RNA Extraction and Histology
  • 04:20RNA Isolation from Pooled Cryosections
  • 06:17Results: RNA Extraction and Histology of Cardiotoxin Injured Muscle
  • 07:40Conclusion

Summary

Traduzione automatica

Traduzione automatica

连续的低温部分被收集，以便利用邻近地区从一个单一的小鼠骨骼肌基因表达测量组织的应用程序和RNA的富集。 30毫克汇集冷冻切片和测量的应用程序之间直接比较 - 高品质的RNA是从20获得。

Tags

CryosectioningContiguous RegionsSingle Mouse Skeletal MuscleGene ExpressionHistological AnalysesRegenerative EffectsMuscular InjuryViral Gene DeliveryIntramuscular InjectionHistological DataMolecular DataEmbedding Resin2-MethylbutaneLiquid NitrogenCryostatRNA ExtractionTibialis Anterior

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