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Spatiotemporal Analysis of Cytokinetic Events in Fission Yeast
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Biologia
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JoVE Journal Biologia
Spatiotemporal Analysis of Cytokinetic Events in Fission Yeast

Spatiotemporal Analysis of Cytokinetic Events in Fission Yeast

DOI:
10.3791/55109-v

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11:19 min

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February 20, 2017

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, , ,
1Department of Biochemistry & Cellular and Molecular Biology,University of Tennessee

Capitoli

  • 00:05Titolo
  • 00:46Sample Preparation
  • 01:40Image Acquisition
  • 03:25Image Analysis: Temporal Analysis of Cytokinetic Events
  • 06:30Image Analysis: Spatial Analysis of Cytokinetic Events
  • 08:48Results: Analysis of Cytokinetic Events by Live-cell Imaging
  • 10:36Conclusion

Summary

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Traduzione automatica

The fission yeast, Schizosaccharomyces pombe is an excellent model system to study cytokinesis, the final stage in cell division. Here we describe a microscopy approach to analyze different cytokinetic events in live fission yeast cells.

Tags

Keywords: Spatiotemporal AnalysisCytokinesisFission YeastLive Cell MicroscopyCell CycleDICGFPRFPZ-seriesTime-lapse Imaging

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