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En simpel en-trin Dissektion Protokol for Whole-mount Forberedelse af Voksen<em> Drosophila</em> Brains
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1Center for Metabolic and Degenerative Diseases, The Brown Foundation Institute of Molecular Medicine, Programs in Human and Molecular Genetics and Neuroscience, The Graduate School of Biomedical Sciences at Houston,The University of Texas Health Science Center at Houston, 2Department of Natural Sciences,University of Houston – Downtown, 3Department of Neurobiology and Anatomy, McGovern Medical School,The University of Texas Health Science Center at Houston
Capitoli
- 00:05Titolo
- 01:12Microsurgical Dissection of Adult Fly Heads
- 02:56Immunofluorescent Staining of the Brains
- 04:44Mounting and Imaging of Brain Tissue
- 06:02Results: Anti-TH Staining in Dissected Fly Brains Allows Quantification of DA Neurons
- 07:40Conclusion
Den voksne Drosophila hjerne er et værdifuldt system til at studere neuronal kredsløb, højere hjernefunktioner, og komplekse lidelser. En effektiv metode til at dissekere hele hjernevæv fra den lille flue hoved vil lette hjerne-baserede undersøgelser. Her beskriver vi en enkel, et-trins dissektion protokol af voksne hjerner med velbevaret morfologi.
