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In Vitro Differentiation of Mature Myofibers for Live Imaging
JoVE Journal
Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
In Vitro Differentiation of Mature Myofibers for Live Imaging

In Vitro Differentiation of Mature Myofibers for Live Imaging

DOI:
10.3791/55141-v

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08:12 min

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January 07, 2017

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, , ,
1Instituto de Medicina Molecular, Faculdade de Medicina,Universidade de Lisboa, 2Myology Research Center, UM76-INSERM U974-CNRS FRE 361,Sorbonne University, UPMC University of Paris 6

Capitoli

  • 00:05Titolo
  • 00:46Neonatal Mouse Hind-limb Dissection
  • 02:09Myoblast Isolation
  • 04:09Myofiber Differentiation and Immunostaining
  • 06:10Results: Developmental Stages of the Myoblast Culture
  • 07:21Conclusion

Summary

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Traduzione automatica

Muscle cells are among the most complex eukaryotic cells. We present a protocol for the in vitro differentiation of highly mature myofibers that allows for genetic manipulation and clear imaging during all developmental stages.

Tags

In Vitro DifferentiationMature MyofibersLive ImagingNeonatal Mouse MyoblastsTransverse Shear TriadSarcomere AssemblyMyofibril AssemblyOrganelle PositioningTime-lapse MicroscopyEnzymatic DigestionCell IsolationFibroblast AdhesionBasement Membrane Coating

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