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Método para visualizar e analisar proteínas de interação de membrana por microscopia eletrônica de transmissão
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Capitoli
- 00:05Titolo
- 01:08Preparation, Reconstitution, and Analysis of Empty Nanodiscs
- 05:11Preparation and Analysis of the Nanodisc-Monotopic Protein Complex
- 07:56Results: Monotopic Membrane Protein Binding on the Surfaces of Empty Nanodiscs (NDs)
- 09:26Conclusion
Many proteins perform their function when attached to membrane surfaces. The binding of extrinsic proteins on nanodisc membranes can be indirectly imaged by transmission electron microscopy. We show that the characteristic stacking (rouleau) of nanodiscs induced by the negative stain sodium phosphotungstate is prevented by the binding of extrinsic protein.
