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Capitoli
- 00:05Titolo
- 00:40Collection of Cells from Culture
- 01:34Fixation
- 02:31Propidium Iodide Staining
- 03:39Sorting
- 05:32DNA Sonication
- 06:19Results: Measurements of S/G1 DNA Content Produce Detailed Time of Replication Maps
- 07:34Conclusion
We describe here a relatively fast and simple approach for mapping genome-wide mammalian replication timing, from cell isolation to the basic analysis of the sequencing results. A genomic map of a representative replication program will be provided following the protocol.
