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JoVE Journal Neuroscience
Preparation of Horizontal Slices of Adult Mouse Retina for Electrophysiological Studies
 

Preparation of Horizontal Slices of Adult Mouse Retina for Electrophysiological Studies

Article DOI: 10.3791/55173-v • 07:02 min • January 27th, 2017
January 27th, 2017 •
1, 1
1Department of Biology, Friedrich-Alexander-University (FAU) Erlangen-Nürnberg

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We developed a horizontal slice preparation of the adult mammalian retina with the plane of sectioning parallel to the retinal surface. Dendritic fields of nonradially oriented retinal neurons were not truncated, allowing the study of signal processing with patch-clamp and imaging techniques.

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Keywords: Horizontal Slices Adult Mouse Retina Electrophysiological Studies Retinal Neurons Ribbon Synapses Visual Information Processing Horizontal Cells Amacrine Cells Eyeball Dissection Cornea Removal Vitreous Body Removal Retina Isolation Retinal Tissue Preparation
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