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成年小鼠视网膜电生理研究水平切片的制备

JoVE Journal
Neuroscienze

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JoVE Journal Neuroscienze

Preparation of Horizontal Slices of Adult Mouse Retina for Electrophysiological Studies

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成年小鼠视网膜电生理研究水平切片的制备

DOI:

10.3791/55173-v

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07:02 min

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January 27, 2017

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Andreas Feigenspan, Norbert Zsolt Babai

¹Department of Biology,Friedrich-Alexander-University (FAU) Erlangen-Nürnberg

Capitoli

00:05Titolo
00:57Dissection of Mouse Retina
03:12Embedding of Retinal Pieces in Agarose
04:14Mounting of Retinal Pieces
04:58Results: Synaptic Activity Recorded from a Horizontal Cell Body
06:24Conclusion

Summary

Traduzione automatica

我们开发了成年哺乳动物视网膜的水平切片准备具有平行切片于视网膜表面的平面。非径向导向的视网膜神经元树突领域并没有截断，使信号处理与膜片钳和成像技术研究。

Tags

Keywords: Horizontal Slices Adult Mouse Retina Electrophysiological Studies Retinal Neurons Ribbon Synapses Visual Information Processing Horizontal Cells Amacrine Cells Eyeball Dissection Cornea Removal Vitreous Body Removal Retina Isolation

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