Rivista
/
Neuroscienze
/
Analyzing Synaptic Modulation of Drosophila melanogaster Photoreceptors after Exposure to Prolonged Light
JoVE Journal
Neuroscienze
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Neuroscienze
Analyzing Synaptic Modulation of Drosophila melanogaster Photoreceptors after Exposure to Prolonged Light

Analyzing Synaptic Modulation of Drosophila melanogaster Photoreceptors after Exposure to Prolonged Light

DOI:
10.3791/55176-v

•

11:36 min

•

February 10, 2017

•

, , , , ,
1Department of Neuroscience of Disease, Center for Transdisciplinary Research,Niigata University, 2Brain Research Institute,Niigata University, 3Image and Data Analysis Facility,German Center for Neurodegenerative Diseases (DZNE), 4Graduate School of Life Science and Technology,Tokyo Institute of Technology (Titech), 5Dendrite Differentiation,German Center for Neurodegenerative Diseases (DZNE)

Capitoli

  • 00:05Titolo
  • 00:47Treatment of Flies and Imaging Brains
  • 02:54Image Analysis: Generating the Spots, Surface Objects, Start-points, and End-points
  • 08:04Image Analysis: Calculating the Number of Spots, Distribution of Spot Frequency and Enrichment Level of Brp-GFP
  • 09:48Results: Brp-GFP Expression in R8 Synapses of the Medulla
  • 11:02Conclusion

Summary

Traduzione automatica

Traduzione automatica

Here we show how to quantify the number and spatial distribution of synaptic active zones in Drosophila melanogaster photoreceptors, highlighted with a genetically encoded molecular marker, and their modulation after prolonged exposure to light.

Tags

Keywords: Synaptic ModulationDrosophila MelanogasterPhotoreceptorsProlonged Light ExposureSynaptic PlasticitySynaptic DynamicsNeuron ActivitySynaptic AnalysisBrp ExpressionR7 And R8 Photoreceptor AxonsImmunolabeling3D ImagingImage AnalysisSpot Detection

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

check_url/it/55176?article_type=v

In vivo Visualization of Synaptic Vesicles Within Drosophila Larval Segmental Axons
check_url/it/55176?article_type=v

Optogenetic Stimulation of Escape Behavior in Drosophila melanogaster
check_url/it/55176?article_type=v

Determination of the Spontaneous Locomotor Activity in Drosophila melanogaster
check_url/it/55176?article_type=v

Conditions Affecting Social Space in Drosophila melanogaster
check_url/it/55176?article_type=v

High Throughput Assay to Examine Egg-Laying Preferences of Individual Drosophila melanogaster
check_url/it/55176?article_type=v

Electrophysiological Method for Recording Intracellular Voltage Responses of Drosophila Photoreceptors and Interneurons to Light Stimuli In Vivo
check_url/it/55176?article_type=v

Electrophysiological Method for Whole-cell Voltage Clamp Recordings from Drosophila Photoreceptors
check_url/it/55176?article_type=v

Desensitization and Recovery of Crayfish Photoreceptors Upon Delivery of a Light Stimulus
check_url/it/55176?article_type=v

Monitoring Cell-to-cell Transmission of Prion-like Protein Aggregates in Drosophila Melanogaster
check_url/it/55176?article_type=v

Metabolic Analysis of Drosophila melanogaster Larval and Adult Brains

Read Article