Rivista
/
/
Gerichte plasmamembraan Levering van een hydrofobe Cargo ingekapseld in een Liquid Crystal nanodeeltjes Carrier
JoVE Journal
Bioingegneria
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Bioingegneria
Targeted Plasma Membrane Delivery of a Hydrophobic Cargo Encapsulated in a Liquid Crystal Nanoparticle Carrier
DOI:

10:16 min

February 08, 2017

, , , ,

Capitoli

  • 00:05Titolo
  • 00:51Conjugation of PEG-Chol to DiO-LCNP via EDC Coupling and Characterization of the Product
  • 02:33Preparation of Cell Culture Dishes for Delivery Experiments and Imaging
  • 04:07Cellular Delivery of DiO and DiO-LCNPs and Imaging of Fixed Cells
  • 06:11Cellular Delivery DiO-LCNPs and DiI FRET Imaging in Live Cells
  • 07:37Results: Analysis of DiO-LCNP-PEG-Chol Cellular Uptake to the Plasma Membrane and Quantification of Cytotoxicity
  • 08:57Conclusion

Summary

Traduzione automatica

Vloeibaar kristal nanodeeltje (LCNP) Nanodrager wordt benut als middel voor de gecontroleerde afgifte van een hydrofoob lading naar de plasmamembraan van levende cellen.

Video correlati

Read Article