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Targeted Plasma Membrane Livraison d'un Hydrophobe Cargo encapsulé dans un cristal liquide Nanoparticules Transporteur
JoVE Journal
Bioingegneria
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JoVE Journal Bioingegneria
Targeted Plasma Membrane Delivery of a Hydrophobic Cargo Encapsulated in a Liquid Crystal Nanoparticle Carrier
DOI:

10:16 min

February 08, 2017

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Capitoli

  • 00:05Titolo
  • 00:51Conjugation of PEG-Chol to DiO-LCNP via EDC Coupling and Characterization of the Product
  • 02:33Preparation of Cell Culture Dishes for Delivery Experiments and Imaging
  • 04:07Cellular Delivery of DiO and DiO-LCNPs and Imaging of Fixed Cells
  • 06:11Cellular Delivery DiO-LCNPs and DiI FRET Imaging in Live Cells
  • 07:37Results: Analysis of DiO-LCNP-PEG-Chol Cellular Uptake to the Plasma Membrane and Quantification of Cytotoxicity
  • 08:57Conclusion

Summary

Traduzione automatica

Un cristal liquide nanoparticule (LCNP) nanocarrier est exploitée en tant que véhicule pour la libération contrôlée d'une cargaison hydrophobe à la membrane plasmique de cellules vivantes.

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