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3D Magnetic Stem Cell Aggregation and Bioreactor Maturation for Cartilage Regeneration
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Capitoli
- 00:05Titolo
- 01:05Magnetic Device Construction and Stem Cell Labeling
- 02:45Magnetic Cell Seeding
- 04:59Chondrocytes Differentiation
- 07:24Results: Representative Cartilage Regeneration Analyses
- 08:28Conclusion
Chondrogenesis from stem cells requires fine tuning the culture conditions. Here, we present a magnetic approach for condensing cells, an essential step to initiate chondrogenesis. In addition, we show that dynamic maturation in a bioreactor applies mechanical stimulation to the cellular constructs and enhances cartilaginous extracellular matrix production.
Tags
3D Magnetic Stem Cell AggregationCartilage RegenerationMesenchymal Stromal CellsCell CondensationCellular BioreactorDynamic MaturationMuscle RepairCardiac RepairVascular RepairMicromagnet DeviceScaffold SeedingIron Oxide NanoparticlesMagnetic Cell LabelingCell AggregationCell CultureTrypsin-EDTACell PelletCell CountingGlass Bottom Petri Dish
