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MitoCeption：转让人离MSC线粒体脑胶质瘤干细胞

JoVE Journal
Biologia

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JoVE Journal Biologia

MitoCeption: Transferring Isolated Human MSC Mitochondria to Glioblastoma Stem Cells

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MitoCeption：转让人离MSC线粒体脑胶质瘤干细胞

DOI:

10.3791/55245-v

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11:13 min

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February 22, 2017

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Brice Nzigou Mombo, Sabine Gerbal-Chaloin, Aleksandra Bokus, Martine Daujat-Chavanieu, Christian Jorgensen, Jean-Philippe Hugnot, Marie-Luce Vignais

¹Institute for Regenerative Medicine and Biotherapy (IRMB), INSERM U1183,Montpellier University, ²Institute of Neurosciences of Montpellier (INM), INSERM U1051,Montpellier University

Capitoli

00:05Titolo
01:09Seeding of Glioblastoma Stem Cells
03:00Mesenchymal Stem Cells Mitochondria Isolation
06:13Transfer of Isolated MSC Mitochondria to GSCs
07:50Results: Isolation and Transfer of Mitochondria into Mesenchymal Stem Cells
09:39Conclusion

Summary

Traduzione automatica

这里，协议（MitoCeption）呈现给传送线粒体，从人类间质干细胞（MSC）隔离，胶质母细胞瘤干细胞（GSC），具有研究它们对GSC代谢和功能的生物效应的目的。类似的协议可以适于其它类型的细胞之间转移线粒体。

Tags

Keywords: Mitochondria Transfer MitoCeption Glioblastoma Stem Cells Mesenchymal Stem Cells Metabolism Tunneling Nanotubes Microvesicles Vital Dyes Cell Labeling Cell Culture Neurospheres Trypsin DNase I Trypsin Inhibitor Cell Counting Cell Seeding Centrifugation Cell Incubation Mitochondria Isolation

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