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MitoCeption: Overføre isolert human MSC Mitokondrier å glioblastom stamceller
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Biologia
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JoVE Journal Biologia
MitoCeption: Transferring Isolated Human MSC Mitochondria to Glioblastoma Stem Cells

MitoCeption: Overføre isolert human MSC Mitokondrier å glioblastom stamceller

DOI:
10.3791/55245-v

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11:13 min

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February 22, 2017

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, , , , , ,
1Institute for Regenerative Medicine and Biotherapy (IRMB), INSERM U1183,Montpellier University, 2Institute of Neurosciences of Montpellier (INM), INSERM U1051,Montpellier University

Capitoli

  • 00:05Titolo
  • 01:09Seeding of Glioblastoma Stem Cells
  • 03:00Mesenchymal Stem Cells Mitochondria Isolation
  • 06:13Transfer of Isolated MSC Mitochondria to GSCs
  • 07:50Results: Isolation and Transfer of Mitochondria into Mesenchymal Stem Cells
  • 09:39Conclusion

Summary

Traduzione automatica

Traduzione automatica

Her er en protokoll (MitoCeption) present å overføre mitokondriene, isolert fra humane stamceller (MSC), til glioblastom stamceller (GSC), med mål om å studere deres biologiske effekter på GSC metabolisme og funksjoner. En lignende protokoll kan tilpasses til å overføre mitokondriene mellom andre celletyper.

Tags

Keywords: Mitochondria TransferMitoCeptionGlioblastoma Stem CellsMesenchymal Stem CellsMetabolismTunneling NanotubesMicrovesiclesVital DyesCell LabelingCell CultureNeurospheresTrypsinDNase ITrypsin InhibitorCell CountingCell SeedingCentrifugationCell IncubationMitochondria Isolation

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