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Isolation of Intact Mitochondria from Skeletal Muscle by Differential Centrifugation for High-resolution Respirometry Measurements
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Biologia
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JoVE Journal Biologia
Isolation of Intact Mitochondria from Skeletal Muscle by Differential Centrifugation for High-resolution Respirometry Measurements

Isolation of Intact Mitochondria from Skeletal Muscle by Differential Centrifugation for High-resolution Respirometry Measurements

DOI:
10.3791/55251-v

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10:25 min

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March 08, 2017

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,
1Department of Intensive Care Medicine,Inselspital, Bern University Hospital

Capitoli

  • 00:05Titolo
  • 01:01Skeletal Muscle Homogenization and Mitochondrial Isolation
  • 04:00High-resolution Respirometry
  • 04:44Complex I-dependent Respiration, Complex II-dependent Respiration, and Complex IV-dependent Respiration
  • 06:51Results: High-resolution Respirometry Measurements from Intact Mitochondria Isolated from Skeletal Muscle
  • 08:35Conclusion

Summary

Traduzione automatica

Traduzione automatica

Here, a quadriceps muscle specimen is taken from an anaesthetized pig and mitochondria are isolated by differential centrifugation. Then, the respiratory rates of mitochondrial respiratory chain complexes I, II and IV are determined using high-resolution respirometry.

Tags

Keywords: Mitochondria IsolationSkeletal MuscleDifferential CentrifugationRespirometryRespiratory Chain ComplexesMitochondrial DysfunctionNeurological DiseasesSepsisAgingBSACentrifugation

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