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Murine Lymphocyte Labeling by 64Cu-Antibody Receptor Targeting for In Vivo Cell Trafficking by PET/CT
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JoVE Journal Immunologia e infezioni
Murine Lymphocyte Labeling by 64Cu-Antibody Receptor Targeting for In Vivo Cell Trafficking by PET/CT

Murine Lymphocyte Labeling by 64Cu-Antibody Receptor Targeting for In Vivo Cell Trafficking by PET/CT

DOI:
10.3791/55270-v

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11:34 min

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April 29, 2017

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, , , , , ,
1Werner Siemens Imaging Center, Department of Preclinical Imaging and Radiopharmacy,Eberhard Karls University Tübingen, 2Department of Dermatology,Eberhard Karls University Tübingen

Capitoli

  • 00:05Titolo
  • 01:13Chicken Ovalbumin-specific TH1 (cOVA-TH1) Cell isolation and Antigen Presenting Cell Co-culture
  • 04:42cOVA-TH1 Cell Radiolabeling and Uptake and Efflux Analysis
  • 06:44Positron Emission Tomograph–Computed Tomography (PET/CT) In Vivo Imaging
  • 08:26Results: Representative cOVA-TH1 Cell Tracking In Vitro and In Vivo
  • 10:32Conclusion

Summary

Traduzione automatica

Traduzione automatica

Following the preparation of a 64Cu-modified monoclonal antibody binding to a transgenic murine T cell receptor, T cells are radiolabeled in vivo, analyzed for viability, functionality, labeling stability and apoptosis, and adoptively transferred into mice with an airway delayed-type hypersensitivity reaction for non-invasive imaging by positron emission tomography/computed tomography (PET/CT).

Tags

Murine Lymphocytes64Cu-antibody ReceptorPET/CTCell TraffickingInflammationCancerCell-based TherapiesRegenerative MedicineCancer ImmunotherapiesCell MigrationCell LabelingChelator ConjugationRadiolabelingLymph NodesSpleenCD4-positive CellsMagnetic Cell Separation

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